3BY7

CRYSTAL STRUCTURE OF A PROTEIN STRUCTURALLY SIMILAR TO SM/LSM-LIKE RNA-BINDING PROTEINS (JCVI_PEP_1096686650277) FROM UNCULTURED MARINE ORGANISM AT 2.60 A RESOLUTION

3BY7 の概要

• エントリーDOI: 10.2210/pdb3by7/pdb
• 分子名称: uncharacterized protein (2 entities in total)
• 機能のキーワード: metagenomics, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, unknown function
• 由来する生物種: uncultured marine organism
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 55737.08

構造登録者

Joint Center for Structural Genomics (JCSG) (登録日: 2008-01-15, 公開日: 2008-01-29, 最終更新日: 2024-10-30)

主引用文献

Das, D.,Kozbial, P.,Axelrod, H.L.,Miller, M.D.,McMullan, D.,Krishna, S.S.,Abdubek, P.,Acosta, C.,Astakhova, T.,Burra, P.,Carlton, D.,Chen, C.,Chiu, H.J.,Clayton, T.,Deller, M.C.,Duan, L.,Elias, Y.,Elsliger, M.A.,Ernst, D.,Farr, C.,Feuerhelm, J.,Grzechnik, A.,Grzechnik, S.K.,Hale, J.,Han, G.W.,Jaroszewski, L.,Jin, K.K.,Johnson, H.A.,Klock, H.E.,Knuth, M.W.,Kumar, A.,Marciano, D.,Morse, A.T.,Murphy, K.D.,Nigoghossian, E.,Nopakun, A.,Okach, L.,Oommachen, S.,Paulsen, J.,Puckett, C.,Reyes, R.,Rife, C.L.,Sefcovic, N.,Sudek, S.,Tien, H.,Trame, C.,Trout, C.V.,van den Bedem, H.,Weekes, D.,White, A.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
Crystal structure of a novel Sm-like protein of putative cyanophage origin at 2.60 A resolution.
Proteins, 75:296-307, 2009

PubMed Abstract: ECX21941 represents a very large family (over 600 members) of novel, ocean metagenome-specific proteins identified by clustering of the dataset from the Global Ocean Sampling expedition. The crystal structure of ECX21941 reveals unexpected similarity to Sm/LSm proteins, which are important RNA-binding proteins, despite no detectable sequence similarity. The ECX21941 protein assembles as a homopentamer in solution and in the crystal structure when expressed in Escherichia coli and represents the first pentameric structure for this Sm/LSm family of proteins, although the actual oligomeric form in vivo is currently not known. The genomic neighborhood analysis of ECX21941 and its homologs combined with sequence similarity searches suggest a cyanophage origin for this protein. The specific functions of members of this family are unknown, but our structure analysis of ECX21941 indicates nucleic acid-binding capabilities and suggests a role in RNA and/or DNA processing.

PubMed: 19173316
DOI: 10.1002/prot.22360

実験手法

X-RAY DIFFRACTION (2.6 Å)