3BWT

Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae

3BWT の概要

• エントリーDOI: 10.2210/pdb3bwt/pdb
• 関連するPDBエントリー: 1IB2 1M6W 1M8X 1M8Y 1M8Z 3BX2 3BX3
• 分子名称: Protein PUF4 (2 entities in total)
• 機能のキーワード: puf4, pumilio, rna binding, ho endonuclease, transcription, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37765.41

構造登録者

Miller, M.T.,Higgin, J.J.,Hall, T.M.T. (登録日: 2008-01-10, 公開日: 2008-03-11, 最終更新日: 2024-02-21)

主引用文献

Miller, M.T.,Higgin, J.J.,Hall, T.M.T.
Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p
Nat.Struct.Mol.Biol., 15:397-402, 2008

PubMed Abstract: Pumilio/FBF (PUF) family proteins are found in eukaryotic organisms and regulate gene expression post-transcriptionally by binding to sequences in the 3' untranslated region of target transcripts. PUF proteins contain an RNA binding domain that typically comprises eight alpha-helical repeats, each of which recognizes one RNA base. Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases. Here we report the crystal structures of Puf4p alone and in complex with a 9-nucleotide (nt) target RNA sequence, revealing that Puf4p accommodates an 'extra' nucleotide by modest adaptations allowing one base to be turned away from the RNA binding surface. Using structural information and sequence comparisons, we created a mutant Puf4p protein that preferentially binds to an 8-nt target RNA sequence over a 9-nt sequence and restores binding of each protein repeat to one RNA base.

PubMed: 18327269
DOI: 10.1038/nsmb.1390

実験手法

X-RAY DIFFRACTION (2.69 Å)