3BWS

Crystal structure of the leptospiral antigen Lp49

3BWS の概要

• エントリーDOI: 10.2210/pdb3bws/pdb
• 分子名称: protein Lp49, SULFATE ION (3 entities in total)
• 機能のキーワード: two-domain, immunoglobulin-like, 7-bladed beta propeller, unknown function
• 由来する生物種: Leptospira interrogans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99474.16

構造登録者

Giuseppe, P.O.,Neves, F.O.,Nascimento, A.L.T.O.,Guimaraes, B.G. (登録日: 2008-01-10, 公開日: 2008-10-07, 最終更新日: 2024-11-06)

主引用文献

Giuseppe, P.O.,Neves, F.O.,Nascimento, A.L.T.O.,Guimaraes, B.G.
The leptospiral antigen Lp49 is a two-domain protein with putative protein binding function
J.Struct.Biol., 163:53-60, 2008

PubMed Abstract: Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Currently available vaccines have limited effectiveness and therapeutic interventions are complicated by the difficulty in making an early diagnosis of leptospirosis. The genome of Leptospira interrogans was recently sequenced and comparative genomic analysis contributed to the identification of surface antigens, potential candidates for development of new vaccines and serodiagnosis. Lp49 is a membrane-associated protein recognized by antibodies present in sera from early and convalescent phases of leptospirosis patients. Its crystal structure was determined by single-wavelength anomalous diffraction using selenomethionine-labelled crystals and refined at 2.0 A resolution. Lp49 is composed of two domains and belongs to the all-beta-proteins class. The N-terminal domain folds in an immunoglobulin-like beta-sandwich structure, whereas the C-terminal domain presents a seven-bladed beta-propeller fold. Structural analysis of Lp49 indicates putative protein-protein binding sites, suggesting a role in Leptospira-host interaction. This is the first crystal structure of a leptospiral antigen described to date.

PubMed: 18508281
DOI: 10.1016/j.jsb.2008.04.003

実験手法

X-RAY DIFFRACTION (1.99 Å)