3BWP
Crystal structure of a self-spliced group II intron
Summary for 3BWP
| Entry DOI | 10.2210/pdb3bwp/pdb |
| Descriptor | Group IIC intron, MAGNESIUM ION, POTASSIUM ION, ... (4 entities in total) |
| Functional Keywords | rna, ribonucleic acid, intron, group ii |
| Total number of polymer chains | 1 |
| Total formula weight | 133900.49 |
| Authors | Toor, N.,Keating, K.S.,Taylor, S.D.,Pyle, A.M. (deposition date: 2008-01-10, release date: 2008-04-15, Last modification date: 2024-02-21) |
| Primary citation | Toor, N.,Keating, K.S.,Taylor, S.D.,Pyle, A.M. Crystal structure of a self-spliced group II intron Science, 320:77-82, 2008 Cited by PubMed Abstract: Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor. PubMed: 18388288DOI: 10.1126/science.1153803 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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