3BWP

Crystal structure of a self-spliced group II intron

3BWP の概要

• エントリーDOI: 10.2210/pdb3bwp/pdb
• 分子名称: Group IIC intron, MAGNESIUM ION, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: rna, ribonucleic acid, intron, group ii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 133900.49

構造登録者

Toor, N.,Keating, K.S.,Taylor, S.D.,Pyle, A.M. (登録日: 2008-01-10, 公開日: 2008-04-15, 最終更新日: 2024-02-21)

主引用文献

Toor, N.,Keating, K.S.,Taylor, S.D.,Pyle, A.M.
Crystal structure of a self-spliced group II intron
Science, 320:77-82, 2008

PubMed Abstract: Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.

PubMed: 18388288
DOI: 10.1126/science.1153803

実験手法

X-RAY DIFFRACTION (3.1 Å)