3BVS

Crystal Structure of Bacillus cereus Alkylpurine DNA Glycosylase AlkD

3BVS の概要

• エントリーDOI: 10.2210/pdb3bvs/pdb
• 分子名称: Alkylpurine DNA Glycosylase AlkD (2 entities in total)
• 機能のキーワード: dna glycosylase, heat repeat, alkylpurine, dna repair, hydrolase
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28423.88

構造登録者

Rubinson, E.H.,Eichman, B.F. (登録日: 2008-01-07, 公開日: 2008-07-15, 最終更新日: 2024-02-21)

主引用文献

Rubinson, E.H.,Metz, A.H.,O'Quin, J.,Eichman, B.F.
A New Protein Architecture for Processing Alkylation Damaged DNA: The Crystal Structure of DNA Glycosylase AlkD.
J.Mol.Biol., 381:13-23, 2008

PubMed Abstract: DNA glycosylases safeguard the genome by locating and excising chemically modified bases from DNA. AlkD is a recently discovered bacterial DNA glycosylase that removes positively charged methylpurines from DNA, and was predicted to adopt a protein fold distinct from from those of other DNA repair proteins. The crystal structure of Bacillus cereus AlkD presented here shows that the protein is composed exclusively of helical HEAT-like repeats, which form a solenoid perfectly shaped to accommodate a DNA duplex on the concave surface. Structural analysis of the variant HEAT repeats in AlkD provides a rationale for how this protein scaffolding motif has been modified to bind DNA. We report 7mG excision and DNA binding activities of AlkD mutants, along with a comparison of alkylpurine DNA glycosylase structures. Together, these data provide important insight into the requirements for alkylation repair within DNA and suggest that AlkD utilizes a novel strategy to manipulate DNA in its search for alkylpurine bases.

PubMed: 18585735
DOI: 10.1016/j.jmb.2008.05.078

実験手法

X-RAY DIFFRACTION (2.1 Å)