3BSN

Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA

3BSN の概要

• エントリーDOI: 10.2210/pdb3bsn/pdb
• 関連するPDBエントリー: 1SH0 3BSO
• 分子名称: RNA dependent RNA polymerase, RNA (5'-R(*UP*GP*CP*CP*CP*GP*GP*G)-3'), RNA (5'-R(*UP*GP*CP*CP*CP*GP*GP*GP*(N5M))-3'), ... (7 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, viral replication, antiviral enzyme inhibitor, helicase, hydrolase, nucleotide-binding, nucleotidyltransferase, rna replication, rna-directed rna polymerase, transferase, transferase-rna complex, transferase/rna
• 由来する生物種: Norwalk virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 63370.25

構造登録者

Zamyatkin, D.F.,Ng, K.K.S. (登録日: 2007-12-26, 公開日: 2008-01-08, 最終更新日: 2023-08-30)

主引用文献

Zamyatkin, D.F.,Parra, F.,Alonso, J.M.,Harki, D.A.,Peterson, B.R.,Grochulski, P.,Ng, K.K.
Structural insights into mechanisms of catalysis and inhibition in norwalk virus polymerase.
J.Biol.Chem., 283:7705-7712, 2008

PubMed Abstract: Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex and either the natural substrate CTP or the inhibitor 5-nitrocytidine triphosphate have been determined to 1.8A resolution. These structures reveal a closed conformation of the polymerase that differs significantly from previously determined open structures of calicivirus and picornavirus polymerases. These closed complexes are trapped immediately prior to the nucleotidyl transfer reaction, with the triphosphate group of the nucleotide bound to two manganese ions at the active site, poised for reaction to the 3'-hydroxyl group of the RNA primer. The positioning of the 5-nitrocytidine triphosphate nitro group between the alpha-phosphate and the 3'-hydroxyl group of the primer suggests a novel, general approach for the design of antiviral compounds mimicking natural nucleosides and nucleotides.

PubMed: 18184655
DOI: 10.1074/jbc.M709563200

実験手法

X-RAY DIFFRACTION (1.8 Å)