3BS1

Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with a Novel Mode of Binding

3BS1 の概要

• エントリーDOI: 10.2210/pdb3bs1/pdb
• 分子名称: DNA (5'-D(*DTP*DTP*DTP*DAP*DAP*DCP*DAP*DGP*DTP*DTP*DAP*DAP*DGP*(BRU)P*DAP*DT)-3'), DNA (5'-D(*DAP*DAP*(BRU)P*DAP*DCP*DTP*DTP*DAP*DAP*DCP*DTP*DGP*DTP*DTP*DAP*DA)-3'), Accessory gene regulator protein A, ... (5 entities in total)
• 機能のキーワード: lyttr, agra, response regulator, dna binding domain, activator, cytoplasm, dna-binding, phosphoprotein, transcription, transcription regulation, two-component regulatory system, transcription regulator
• 由来する生物種: Staphylococcus aureus; 詳細
• 細胞内の位置: Cytoplasm: 3BS1
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 22327.71

構造登録者

Sidote, D.J.,Barbieri, C.,Wu, T.,Stock, A.M. (登録日: 2007-12-21, 公開日: 2008-04-08, 最終更新日: 2024-02-21)

主引用文献

Sidote, D.J.,Barbieri, C.M.,Wu, T.,Stock, A.M.
Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with an Unusual Mode of Binding.
Structure, 16:727-735, 2008

PubMed Abstract: The LytTR domain is a DNA-binding motif found within the AlgR/AgrA/LytR family of transcription factors that regulate virulence factor and toxin gene expression in pathogenic bacteria. This previously uncharacterized domain lacks sequence similarity with proteins of known structure. The crystal structure of the DNA-binding domain of Staphylococcus aureus AgrA complexed with a DNA pentadecamer duplex has been determined at 1.6 A resolution. The structure establishes a 10-stranded beta fold for the LytTR domain and reveals its mode of interaction with DNA. Residues within loop regions of AgrA contact two successive major grooves and the intervening minor groove on one face of the oligonucleotide duplex, inducing a substantial bend in the DNA. Loss of DNA binding upon substitution of key interacting residues in AgrA supports the observed binding mode. This mode of protein-DNA interaction provides a potential target for future antimicrobial drug design.

PubMed: 18462677
DOI: 10.1016/j.str.2008.02.011

実験手法

X-RAY DIFFRACTION (1.6 Å)