3BO1
Ribosome-SecY complex
Summary for 3BO1
| Entry DOI | 10.2210/pdb3bo1/pdb |
| Related | 3BO0 |
| EMDB information | 1484 |
| Descriptor | 23S RIBOSOMAL RNA, PREPROTEIN TRANSLOCASE SecY SUBUNIT, PREPROTEIN TRANSLOCASE SecE SUBUNIT, ... (7 entities in total) |
| Functional Keywords | ribosome-secy complex, membrane, protein transport, translocation, transmembrane, transport, ribosome |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 7 |
| Total formula weight | 93122.10 |
| Authors | Akey, C.W.,Menetret, J.F. (deposition date: 2007-12-15, release date: 2008-12-09, Last modification date: 2024-02-21) |
| Primary citation | Menetret, J.F.,Schaletzky, J.,Clemons, W.M.,Osborne, A.R.,Skanland, S.S.,Denison, C.,Gygi, S.P.,Kirkpatrick, D.S.,Park, E.,Ludtke, S.J.,Rapoport, T.A.,Akey, C.W. Ribosome binding of a single copy of the SecY complex: implications for protein translocation Mol.Cell, 28:1083-1092, 2007 Cited by PubMed Abstract: The SecY complex associates with the ribosome to form a protein translocation channel in the bacterial plasma membrane. We have used cryo-electron microscopy and quantitative mass spectrometry to show that a nontranslating E. coli ribosome binds to a single SecY complex. The crystal structure of an archaeal SecY complex was then docked into the electron density maps. In the resulting model, two cytoplasmic loops of SecY extend into the exit tunnel near proteins L23, L29, and L24. The loop between transmembrane helices 8 and 9 interacts with helices H59 and H50 in the large subunit RNA, while the 6/7 loop interacts with H7. We also show that point mutations of basic residues within either loop abolish ribosome binding. We suggest that SecY binds to this primary site on the ribosome and subsequently captures and translocates the nascent chain. PubMed: 18158904DOI: 10.1016/j.molcel.2007.10.034 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (9.6 Å) |
Structure validation
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