3BO1

Ribosome-SecY complex

3BO1 の概要

• エントリーDOI: 10.2210/pdb3bo1/pdb
• 関連するPDBエントリー: 3BO0
• EMDBエントリー: 1484
• 分子名称: 23S RIBOSOMAL RNA, PREPROTEIN TRANSLOCASE SecY SUBUNIT, PREPROTEIN TRANSLOCASE SecE SUBUNIT, ... (7 entities in total)
• 機能のキーワード: ribosome-secy complex, membrane, protein transport, translocation, transmembrane, transport, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 93122.10

構造登録者

Akey, C.W.,Menetret, J.F. (登録日: 2007-12-15, 公開日: 2008-12-09, 最終更新日: 2024-02-21)

主引用文献

Menetret, J.F.,Schaletzky, J.,Clemons, W.M.,Osborne, A.R.,Skanland, S.S.,Denison, C.,Gygi, S.P.,Kirkpatrick, D.S.,Park, E.,Ludtke, S.J.,Rapoport, T.A.,Akey, C.W.
Ribosome binding of a single copy of the SecY complex: implications for protein translocation
Mol.Cell, 28:1083-1092, 2007

PubMed Abstract: The SecY complex associates with the ribosome to form a protein translocation channel in the bacterial plasma membrane. We have used cryo-electron microscopy and quantitative mass spectrometry to show that a nontranslating E. coli ribosome binds to a single SecY complex. The crystal structure of an archaeal SecY complex was then docked into the electron density maps. In the resulting model, two cytoplasmic loops of SecY extend into the exit tunnel near proteins L23, L29, and L24. The loop between transmembrane helices 8 and 9 interacts with helices H59 and H50 in the large subunit RNA, while the 6/7 loop interacts with H7. We also show that point mutations of basic residues within either loop abolish ribosome binding. We suggest that SecY binds to this primary site on the ribosome and subsequently captures and translocates the nascent chain.

PubMed: 18158904
DOI: 10.1016/j.molcel.2007.10.034

実験手法

ELECTRON MICROSCOPY (9.6 Å)