3BNP
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (A1555G Mutant)
Summary for 3BNP
| Entry DOI | 10.2210/pdb3bnp/pdb |
| Related | 3BNL 3BNN 3BNO 3BNQ 3BNR 3BNS 3BNT |
| Descriptor | A site of human mitochondrial ribosome, A chain, A site of human mitochondrial ribosome, B chain, POTASSIUM ION, ... (4 entities in total) |
| Functional Keywords | ribosome, decoding site, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 13780.42 |
| Authors | Kondo, J.,Westhof, E. (deposition date: 2007-12-14, release date: 2008-06-24, Last modification date: 2024-02-21) |
| Primary citation | Kondo, J.,Westhof, E. The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates Nucleic Acids Res., 36:2654-2666, 2008 Cited by PubMed Abstract: The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation. PubMed: 18346970DOI: 10.1093/nar/gkn112 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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