3BNP

Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (A1555G Mutant)

3BNP の概要

• エントリーDOI: 10.2210/pdb3bnp/pdb
• 関連するPDBエントリー: 3BNL 3BNN 3BNO 3BNQ 3BNR 3BNS 3BNT
• 分子名称: A site of human mitochondrial ribosome, A chain, A site of human mitochondrial ribosome, B chain, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: ribosome, decoding site, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13780.42

構造登録者

Kondo, J.,Westhof, E. (登録日: 2007-12-14, 公開日: 2008-06-24, 最終更新日: 2024-02-21)

主引用文献

Kondo, J.,Westhof, E.
The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates
Nucleic Acids Res., 36:2654-2666, 2008

PubMed Abstract: The A site of the small ribosomal subunit participates in the fidelity of decoding by switching between two states, a resting 'off' state and an active decoding 'on' state. Eight crystal structures of RNA duplexes containing two minimal decoding A sites of the Homo sapiens mitochondrial wild-type, the A1555G mutant or bacteria have been solved. The resting 'off' state of the mitochondrial wild-type A site is surprisingly different from that of the bacterial A site. The mitochondrial A1555G mutant has two types of the 'off' states; one is similar to the mitochondrial wild-type 'off' state and the other is similar to the bacterial 'off' state. Our present results indicate that the dynamics of the A site in bacteria and mitochondria are different, a property probably related to the small number of tRNAs used for decoding in mitochondria. Based on these structures, we propose a hypothesis for the molecular mechanism of non-syndromic hearing loss due to the mitochondrial A1555G mutation.

PubMed: 18346970
DOI: 10.1093/nar/gkn112

実験手法

X-RAY DIFFRACTION (2.7 Å)