3BL2

Crystal Structure of M11, the BCL-2 Homolog of Murine Gamma-herpesvirus 68, Complexed with Mouse Beclin1 (residues 106-124)

3BL2 の概要

• エントリーDOI: 10.2210/pdb3bl2/pdb
• 分子名称: V-bcl-2, Beclin-1 (3 entities in total)
• 機能のキーワード: protein-protein complex, viral bcl-2, beclin1, apoptosis, m11, autophagy, antiviral defense, coiled coil, cytoplasm, golgi apparatus, membrane, viral protein-apoptosis complex, viral protein/apoptosis
• 由来する生物種: Murid herpesvirus 4 (Murine herpesvirus 68); 詳細
• 細胞内の位置: Cytoplasm . Beclin-1-C 35 kDa: Mitochondrion . Beclin-1-C 37 kDa: Mitochondrion : O88597
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34789.75

構造登録者

Oh, B.-H.,Woo, J.-S.,Ku, B. (登録日: 2007-12-10, 公開日: 2008-02-12, 最終更新日: 2023-11-01)

主引用文献

Ku, B.,Woo, J.-S.,Liang, C.,Lee, K.-H.,Hong, H.-S.,E, X.,Kim, K.-S.,Jung, J.U.,Oh, B.-H.
Structural and Biochemical Bases for the Inhibition of Autophagy and Apoptosis by Viral BCL-2 of Murine gamma-Herpesvirus 68
Plos Pathog., 4:e25-e25, 2008

PubMed Abstract: All gammaherpesviruses express homologues of antiapoptotic B-cell lymphoma-2 (BCL-2) to counter the clearance of infected cells by host antiviral defense machineries. To gain insights into the action mechanisms of these viral BCL-2 proteins, we carried out structural and biochemical analyses on the interactions of M11, a viral BCL-2 of murine gamma-herpesvirus 68, with a fragment of proautophagic Beclin1 and BCL-2 homology 3 (BH3) domain-containing peptides derived from an array of proapoptotic BCL-2 family proteins. Mainly through hydrophobic interactions, M11 bound the BH3-like domain of Beclin1 with a dissociation constant of 40 nanomole, a markedly tighter affinity compared to the 1.7 micromolar binding affinity between cellular BCL-2 and Beclin1. Consistently, M11 inhibited autophagy more efficiently than BCL-2 in NIH3T3 cells. M11 also interacted tightly with a BH3 domain peptide of BAK and those of the upstream BH3-only proteins BIM, BID, BMF, PUMA, and Noxa, but weakly with that of BAX. These results collectively suggest that M11 potently inhibits Beclin1 in addition to broadly neutralizing the proapoptotic BCL-2 family in a similar but distinctive way from cellular BCL-2, and that the Beclin1-mediated autophagy may be a main target of the virus.

PubMed: 18248095
DOI: 10.1371/journal.ppat.0040025

実験手法

X-RAY DIFFRACTION (2.3 Å)