3BIX

Crystal structure of the extracellular esterase domain of Neuroligin-1

3BIX の概要

• エントリーDOI: 10.2210/pdb3bix/pdb
• 関連するPDBエントリー: 3BIW
• 分子名称: Neuroligin-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, NICKEL (II) ION, ... (5 entities in total)
• 機能のキーワード: esterase domain, alpha-beta hydrolase, cell adhesion, cell junction, glycoprotein, membrane, postsynaptic cell membrane, synapse, transmembrane
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 259671.85

構造登録者

Arac, D.,Boucard, A.A.,Ozkan, E.,Strop, P.,Newell, E.,Sudhof, T.C.,Brunger, A.T. (登録日: 2007-12-01, 公開日: 2007-12-18, 最終更新日: 2024-10-16)

主引用文献

Arac, D.,Boucard, A.A.,Ozkan, E.,Strop, P.,Newell, E.,Sudhof, T.C.,Brunger, A.T.
Structures of Neuroligin-1 and the Neuroligin-1/Neurexin-1beta Complex Reveal Specific Protein-Protein and Protein-Ca(2+) Interactions.
Neuron, 56:992-1003, 2007

PubMed Abstract: Neurexins and neuroligins provide trans-synaptic connectivity by the Ca2+-dependent interaction of their alternatively spliced extracellular domains. Neuroligins specify synapses in an activity-dependent manner, presumably by binding to neurexins. Here, we present the crystal structures of neuroligin-1 in isolation and in complex with neurexin-1 beta. Neuroligin-1 forms a constitutive dimer, and two neurexin-1 beta monomers bind to two identical surfaces on the opposite faces of the neuroligin-1 dimer to form a heterotetramer. The neuroligin-1/neurexin-1 beta complex exhibits a nanomolar affinity and includes a large binding interface that contains bound Ca2+. Alternatively spliced sites in neurexin-1 beta and in neuroligin-1 are positioned nearby the binding interface, explaining how they regulate the interaction. Structure-based mutations of neuroligin-1 at the interface disrupt binding to neurexin-1 beta, but not the folding of neuroligin-1 and confirm the validity of the binding interface of the neuroligin-1/neurexin-1 beta complex. Our results provide molecular insights for understanding the role of cell-adhesion proteins in synapse function.

PubMed: 18093522
DOI: 10.1016/j.neuron.2007.12.002

実験手法

X-RAY DIFFRACTION (1.8 Å)