3BGF

X-ray crystal structure of the SARS coronavirus spike receptor binding domain in complex with F26G19 Fab

3BGF の概要

• エントリーDOI: 10.2210/pdb3bgf/pdb
• 関連するPDBエントリー: 2AJF 2DD8 2GHV 2GHW
• 分子名称: Spike protein S1, F26G19 Fab, ... (4 entities in total)
• 機能のキーワード: antigen-antibody complex, envelope protein, glycoprotein, host-virus interaction, membrane, transmembrane, virion, virulence, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: SARS coronavirus (SARS-CoV); 詳細
• 細胞内の位置: Virion membrane ; Single-pass type I membrane protein : P59594
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 137889.93

構造登録者

Pak, J.E.,Rini, J.M. (登録日: 2007-11-26, 公開日: 2008-12-02, 最終更新日: 2024-10-30)

主引用文献

Pak, J.E.,Sharon, C.,Satkunarajah, M.,Auperin, T.C.,Cameron, C.M.,Kelvin, D.J.,Seetharaman, J.,Cochrane, A.,Plummer, F.A.,Berry, J.D.,Rini, J.M.
Structural insights into immune recognition of the severe acute respiratory syndrome coronavirus S protein receptor binding domain.
J.Mol.Biol., 388:815-823, 2009

PubMed Abstract: The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for host cell attachment and fusion of the viral and host cell membranes. Within S the receptor binding domain (RBD) mediates the interaction with angiotensin-converting enzyme 2 (ACE2), the SARS-CoV host cell receptor. Both S and the RBD are highly immunogenic and both have been found to elicit neutralizing antibodies. Reported here is the X-ray crystal structure of the RBD in complex with the Fab of a neutralizing mouse monoclonal antibody, F26G19, elicited by immunization with chemically inactivated SARS-CoV. The RBD-F26G19 Fab complex represents the first example of the structural characterization of an antibody elicited by an immune response to SARS-CoV or any fragment of it. The structure reveals that the RBD surface recognized by F26G19 overlaps significantly with the surface recognized by ACE2 and, as such, suggests that F26G19 likely neutralizes SARS-CoV by blocking the virus-host cell interaction.

PubMed: 19324051
DOI: 10.1016/j.jmb.2009.03.042

実験手法

X-RAY DIFFRACTION (3 Å)