3BE6

Crystal structure of FitE (crystal form 2), a group III periplasmic siderophore binding protein

3BE6 の概要

• エントリーDOI: 10.2210/pdb3be6/pdb
• 関連するPDBエントリー: 3BE5
• 分子名称: Putative iron compound-binding protein of ABC transporter family, CHLORIDE ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: open form, closed form, group iii periplasmic binding protein, structural genomics, montreal-kingston bacterial structural genomics initiative, bsgi, metal transport
• 由来する生物種: Escherichia coli O157:H7 EDL933
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131198.91

構造登録者

Shi, R.,Matte, A.,Cygler, M.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2007-11-16, 公開日: 2008-10-28, 最終更新日: 2024-10-30)

主引用文献

Shi, R.,Proteau, A.,Wagner, J.,Cui, Q.,Purisima, E.O.,Matte, A.,Cygler, M.
Trapping open and closed forms of FitE-A group III periplasmic binding protein.
Proteins, 75:598-609, 2008

PubMed Abstract: Periplasmic binding proteins (PBPs) are essential components of bacterial transport systems, necessary for bacterial growth and survival. The two-domain structures of PBPs are topologically classified into three groups based on the number of crossovers or hinges between the globular domains: group I PBPs have three connections, group II have two, and group III have only one. Although a large number of structures for group I or II PBPs are known, fewer group III PBPs have been structurally characterized. Group I and II PBPs exhibit significant domain motions during transition from the unbound to ligand-bound form, however, no large conformational changes have been observed to date in group III PBPs. We have solved the crystal structure of a periplasmic binding protein FitE, part of an iron transport system, fit, recently identified in a clinical E. coli isolate. The structure, determined at 1.8 A resolution, shows that FitE is a group III PBP containing a single alpha-helix bridging the two domains. Among the individual FitE molecules present in two crystal forms we observed three different conformations (open, closed, intermediate). Our crystallographic and molecular dynamics results strongly support the notion that group III PBPs also adopt the same Venus flytrap mechanism as do groups I and II PBPs. Unlike other group III PBPs, FitE forms dimers both in solution and in the crystals. The putative siderophore binding pocket is lined with arginine residues, suggesting an anionic nature of the iron-containing siderophore.

PubMed: 19004000
DOI: 10.1002/prot.22272

実験手法

X-RAY DIFFRACTION (1.82 Å)