3BD9

human 3-O-sulfotransferase isoform 5 with bound PAP

3BD9 の概要

• エントリーDOI: 10.2210/pdb3bd9/pdb
• 分子名称: Heparan sulfate glucosamine 3-O-sulfotransferase 5, ADENOSINE-3'-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: 3-o-sulfotransferase, heparan sulfate, heparan sulfate biosynthesis, substrate specificity, glycoprotein, golgi apparatus, membrane, signal-anchor, transmembrane, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Golgi apparatus membrane; Single-pass type II membrane protein (Probable): Q8IZT8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33396.25

構造登録者

Xu, D.,Moon, A.F.,Song, D.,Liu, J.,Pedersen, L.C. (登録日: 2007-11-14, 公開日: 2008-01-29, 最終更新日: 2024-10-09)

主引用文献

Xu, D.,Moon, A.F.,Song, D.,Pedersen, L.C.,Liu, J.
Engineering sulfotransferases to modify heparan sulfate.
Nat.Chem.Biol., 4:200-202, 2008

PubMed Abstract: The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.

PubMed: 18223645
DOI: 10.1038/nchembio.66

実験手法

X-RAY DIFFRACTION (2.3 Å)