3BCY

Crystal structure of YER067W

3BCY の概要

• エントリーDOI: 10.2210/pdb3bcy/pdb
• 分子名称: Protein YER067W (2 entities in total)
• 機能のキーワード: mixed alpha-helix/beta-sheet fold, unknown function
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Cell membrane; Peripheral membrane protein: P40043
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18265.13

構造登録者

Kozlov, G.,Gehring, K. (登録日: 2007-11-13, 公開日: 2008-11-18, 最終更新日: 2024-11-20)

主引用文献

Domitrovic, T.,Kozlov, G.,Freire, J.C.,Masuda, C.A.,da Silva Almeida, M.,Montero-Lomeli, M.,Atella, G.C.,Matta-Camacho, E.,Gehring, K.,Kurtenbach, E.
Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance.
Plos One, 5:e11163-e11163, 2010

PubMed Abstract: The genome of Saccharomyces cerevisiae is arguably the best studied eukaryotic genome, and yet, it contains approximately 1000 genes that are still relatively uncharacterized. As the majority of these ORFs have no homologs with characterized sequence or protein structure, traditional sequence-based approaches cannot be applied to deduce their biological function. Here, we characterize YER067W, a conserved gene of unknown function that is strongly induced in response to many stress conditions and repressed in drug resistant yeast strains. Gene expression patterns of YER067W and its paralog YIL057C suggest an involvement in energy metabolism. We show that yeast lacking YER067W display altered levels of reserve carbohydrates and a growth deficiency in media that requires aerobic metabolism. Impaired mitochondrial function and overall reduction of ergosterol content in the YER067W deleted strain explained the observed 2- and 4-fold increase in resistance to the drugs fluconazole and amphotericin B, respectively. Cell fractionation and immunofluorescence microscopy revealed that Yer067w is associated with cellular membranes despite the absence of a transmembrane domain in the protein. Finally, the 1.7 A resolution crystal structure of Yer067w shows an alpha-beta fold with low similarity to known structures and a putative functional site.YER067W's involvement with aerobic energetic metabolism suggests the assignment of the gene name RGI1, standing for respiratory growth induced 1. Altogether, the results shed light on a previously uncharacterized protein family and provide basis for further studies of its apparent role in energy metabolism control and drug resistance.

PubMed: 20567505
DOI: 10.1371/journal.pone.0011163

実験手法

X-RAY DIFFRACTION (1.7 Å)