3BBA

Structure of active wild-type Prevotella intermedia interpain A cysteine protease

3BBA の概要

• エントリーDOI: 10.2210/pdb3bba/pdb
• 関連するPDBエントリー: 3BB7
• 分子名称: interpain A (1 entity in total)
• 機能のキーワード: prevotella intermedia, zymogen activation, cysteine protease, hydrolase
• 由来する生物種: Prevotella intermedia
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54698.78

構造登録者

Mallorqui-Fernandez, N.,Manandhar, S.P.,Mallorqui-Fernandez, G.,Uson, I.,Wawrzonek, K.,Kantyka, T.,Sola, M.,Thogersen, I.B.,Enghild, J.J.,Potempa, J.,Gomis-Ruth, F.X. (登録日: 2007-11-09, 公開日: 2007-11-20, 最終更新日: 2023-11-01)

主引用文献

Mallorqui-Fernandez, N.,Manandhar, S.P.,Mallorqui-Fernandez, G.,Uson, I.,Wawrzonek, K.,Kantyka, T.,Sola, M.,Thogersen, I.B.,Enghild, J.J.,Potempa, J.,Gomis-Ruth, F.X.
A New Autocatalytic Activation Mechanism for Cysteine Proteases Revealed by Prevotella intermedia Interpain A
J.Biol.Chem., 283:2871-2882, 2008

PubMed Abstract: Prevotella intermedia is a major periodontopathogen contributing to human gingivitis and periodontitis. Such pathogens release proteases as virulence factors that cause deterrence of host defenses and tissue destruction. A new cysteine protease from the cysteine-histidine-dyad class, interpain A, was studied in its zymogenic and self-processed mature forms. The latter consists of a bivalved moiety made up by two subdomains. In the structure of a catalytic cysteine-to-alanine zymogen variant, the right subdomain interacts with an unusual prodomain, thus contributing to latency. Unlike the catalytic cysteine residue, already in its competent conformation in the zymogen, the catalytic histidine is swung out from its active conformation and trapped in a cage shaped by a backing helix, a zymogenic hairpin, and a latency flap in the zymogen. Dramatic rearrangement of up to 20A of these elements triggered by a tryptophan switch occurs during activation and accounts for a new activation mechanism for proteolytic enzymes. These findings can be extrapolated to related potentially pathogenic cysteine proteases such as Streprococcus pyogenes SpeB and Porphyromonas gingivalis periodontain.

PubMed: 17993455
DOI: 10.1074/jbc.M708481200

実験手法

X-RAY DIFFRACTION (3.2 Å)