3B8O

Structure of WzzE- Bacterial Polysaccharide Co-polymerase

3B8O の概要

• エントリーDOI: 10.2210/pdb3b8o/pdb
• 関連するPDBエントリー: 3B8M 3B8N 3B8P
• 分子名称: Lipopolysaccharide biosynthesis protein wzzE (2 entities in total)
• 機能のキーワード: wzz, wzze, bacterial polysaccharide co-polymerase, inner membrane, lipopolysaccharide biosynthesis, membrane, transmembrane, membrane protein, biosynthetic protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane ; Multi-pass membrane protein : P0AG01
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 247311.77

構造登録者

Tocilj, A.,Matte, A.,Cygler, M. (登録日: 2007-11-01, 公開日: 2008-01-22, 最終更新日: 2024-10-16)

主引用文献

Tocilj, A.,Munger, C.,Proteau, A.,Morona, R.,Purins, L.,Ajamian, E.,Wagner, J.,Papadopoulos, M.,Van Den Bosch, L.,Rubinstein, J.L.,Fethiere, J.,Matte, A.,Cygler, M.
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length
Nat.Struct.Mol.Biol., 15:130-138, 2008

PubMed Abstract: The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length distributions to surface polysaccharides. Despite very low sequence similarities, they have a common protomer structure with a long central alpha-helix extending 100 A into the periplasm. The protomers self-assemble into bell-shaped oligomers of variable sizes, with a large internal cavity. Electron microscopy shows that one of the full-length PCPs has a similar organization as that observed in the crystal for its periplasmic domain alone. Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery.

PubMed: 18204465
DOI: 10.1038/nsmb.1374

実験手法

X-RAY DIFFRACTION (2.4 Å)