3B72

Crystal structure of lysozyme folded in SDS and 2-methyl-2,4-pentanediol

3B72 の概要

• エントリーDOI: 10.2210/pdb3b72/pdb
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: hen egg-white lysozyme, protein-sds complex, mpd, allergen, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16587.90

構造登録者

Michaux, C.,Pouyez, J.,Wouters, J.,Prive, G.G. (登録日: 2007-10-30, 公開日: 2008-09-09, 最終更新日: 2024-11-20)

主引用文献

Michaux, C.,Pouyez, J.,Wouters, J.,Prive, G.G.
Protecting role of cosolvents in protein denaturation by SDS: a structural study.
Bmc Struct.Biol., 8:29-29, 2008

PubMed Abstract: Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by addition of the amphipathic solvent 2,4-Methyl-2-PentaneDiol (MPD), used as protecting but also as refolding agent for these proteins. Although the persistence of the protein activity in the SDS/MPD mixture was clearly established, preservation of their structure was only speculative until now.

PubMed: 18522744
DOI: 10.1186/1472-6807-8-29

実験手法

X-RAY DIFFRACTION (1.5 Å)