3B52

Ni,Fe-CODH-600 mV state + CO2

3B52 の概要

• エントリーDOI: 10.2210/pdb3b52/pdb
• 関連するPDBエントリー: 3B51 3B53
• 分子名称: Carbon monoxide dehydrogenase 2, FE (II) ION, IRON/SULFUR CLUSTER, ... (7 entities in total)
• 機能のキーワード: cluster c, 4fe-4s, cytoplasm, iron, iron-sulfur, membrane, metal-binding, nickel, oxidoreductase
• 由来する生物種: Carboxydothermus hydrogenoformans Z-2901
• 細胞内の位置: Cytoplasm: Q9F8A8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 70173.69

構造登録者

Jeoung, J.H.,Dobbek, H. (登録日: 2007-10-25, 公開日: 2007-12-11, 最終更新日: 2024-02-21)

主引用文献

Jeoung, J.H.,Dobbek, H.
Carbon dioxide activation at the Ni,Fe-cluster of anaerobic carbon monoxide dehydrogenase.
Science, 318:1461-1464, 2007

PubMed Abstract: Anaerobic CO dehydrogenases catalyze the reversible oxidation of CO to CO2 at a complex Ni-, Fe-, and S-containing metal center called cluster C. We report crystal structures of CO dehydrogenase II from Carboxydothermus hydrogenoformans in three different states. In a reduced state, exogenous CO2 supplied in solution is bound and reductively activated by cluster C. In the intermediate structure, CO2 acts as a bridging ligand between Ni and the asymmetrically coordinated Fe, where it completes the square-planar coordination of the Ni ion. It replaces a water/hydroxo ligand bound to the Fe ion in the other two states. The structures define the mechanism of CO oxidation and CO2 reduction at the Ni-Fe site of cluster C.

PubMed: 18048691
DOI: 10.1126/science.1148481

実験手法

X-RAY DIFFRACTION (1.5 Å)