3AYF

Crystal structure of nitric oxide reductase

3AYF の概要

• エントリーDOI: 10.2210/pdb3ayf/pdb
• 関連するPDBエントリー: 3AYG
• 分子名称: Nitric oxide reductase, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (8 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 95150.89

構造登録者

Matsumoto, Y.,Tosha, T.,Pisliakov, A.V.,Hino, T.,Sugimoti, H.,Nagano, S.,Sugita, Y.,Shiro, Y. (登録日: 2011-05-06, 公開日: 2012-01-25, 最終更新日: 2024-03-13)

主引用文献

Matsumoto, Y.,Tosha, T.,Pisliakov, A.V.,Hino, T.,Sugimoto, H.,Nagano, S.,Sugita, Y.,Shiro, Y.
Crystal structure of quinol-dependent nitric oxide reductase from Geobacillus stearothermophilus
Nat.Struct.Mol.Biol., 19:238-245, 2012

PubMed Abstract: The structure of quinol-dependent nitric oxide reductase (qNOR) from G. stearothermophilus, which catalyzes the reduction of NO to produce the major ozone-depleting gas N(2)O, has been characterized at 2.5 Å resolution. The overall fold of qNOR is similar to that of cytochrome c-dependent NOR (cNOR), and some structural features that are characteristic of cNOR, such as the calcium binding site and hydrophilic cytochrome c domain, are observed in qNOR, even though it harbors no heme c. In contrast to cNOR, structure-based mutagenesis and molecular dynamics simulation studies of qNOR suggest that a water channel from the cytoplasm can serve as a proton transfer pathway for the catalytic reaction. Further structural comparison of qNOR with cNOR and aerobic and microaerobic respiratory oxidases elucidates their evolutionary relationship and possible functional conversions.

PubMed: 22266822
DOI: 10.1038/nsmb.2213

実験手法

X-RAY DIFFRACTION (2.5 Å)