3AVY

Structure of viral RNA polymerase complex 6

3AVY の概要

• エントリーDOI: 10.2210/pdb3avy/pdb
• 関連するPDBエントリー: 3AVT 3AVU 3AVV 3AVW 3AVX
• 分子名称: Elongation factor Ts, Elongation factor Tu, LINKER, Q beta replicase, RNA (5'-R(*GP*GP*GP*UP*CP*CP*AP*UP*AP*AP*AP*AP*U)-3'), RNA (5'-R(*AP*AP*CP*GP*AP*UP*UP*UP*UP*AP*UP*GP*GP*AP*CP*CP*CP*A)-3'), ... (6 entities in total)
• 機能のキーワード: rna polymerase, translation, transferase-rna complex, transferase/rna
• 由来する生物種: Escherichia coli O157:H7; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 151848.00

構造登録者

Takeshita, D.,Tomita, K. (登録日: 2011-03-08, 公開日: 2012-01-18, 最終更新日: 2023-11-01)

主引用文献

Takeshita, D.,Tomita, K.
Molecular basis for RNA polymerization by Q beta replicase
Nat.Struct.Mol.Biol., 19:229-237, 2012

PubMed Abstract: Core Qβ replicase comprises the Qβ virus-encoded RNA-dependent RNA polymerase (β-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Qβ replicase reveal that at the initiation stage, the 3'-adenine of the template RNA provides a stable platform for de novo initiation. EF-Tu in Qβ replicase forms a template exit channel with the β-subunit. At the elongation stages, the C-terminal region of the β-subunit, assisted by EF-Tu, splits the temporarily double-stranded RNA between the template and nascent RNAs before translocation of the single-stranded template RNA into the exit channel. Therefore, EF-Tu in Qβ replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis.

PubMed: 22245970
DOI: 10.1038/nsmb.2204

実験手法

X-RAY DIFFRACTION (2.616 Å)