3AQZ

Crystal structure of Plodia interpunctella beta-GRP/GNBP3 N-terminal domain with laminarihexaoses

3AQZ の概要

• エントリーDOI: 10.2210/pdb3aqz/pdb
• 関連するPDBエントリー: 2KHA 2RQE 3AQX 3AQY 3IE4
• 分子名称: Beta-1,3-glucan-binding protein, beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: beta-sandwich, immune receptor, beta-1, 3-glucan, sugar binding protein
• 由来する生物種: Plodia interpunctella (Indian meal moth)
• 細胞内の位置: Secreted: Q8MU95
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26335.02

構造登録者

Kanagawa, M.,Satoh, T.,Ikeda, A.,Adachi, Y.,Ohno, N.,Yamaguchi, Y. (登録日: 2010-11-22, 公開日: 2011-06-22, 最終更新日: 2023-11-01)

主引用文献

Kanagawa, M.,Satoh, T.,Ikeda, A.,Adachi, Y.,Ohno, N.,Yamaguchi, Y.
Structural insights into recognition of triple-helical beta-glucans by an insect fungal receptor
J.Biol.Chem., 286:29158-29165, 2011

PubMed Abstract: The innate ability to detect pathogens is achieved by pattern recognition receptors, which recognize non-self-components such as β1,3-glucan. β1,3-Glucans form a triple-helical structure stabilized by interchain hydrogen bonds. β1,3-Glucan recognition protein (βGRP)/gram-negative bacteria-binding protein 3 (GNBP3), one of the pattern recognition receptors, binds to long, structured β1,3-glucan to initiate innate immune response. However, binding details and how specificity is achieved in such receptors remain important unresolved issues. We solved the crystal structures of the N-terminal β1,3-glucan recognition domain of βGRP/GNBP3 (βGRP-N) in complex with the β1,3-linked glucose hexamer, laminarihexaose. In the crystals, three structured laminarihexaoses simultaneously interact through six glucose residues (two from each chain) with one βGRP-N. The spatial arrangement of the laminarihexaoses bound to βGRP-N is almost identical to that of a β1,3-glucan triple-helical structure. Therefore, our crystallographic structures together with site-directed mutagenesis data provide a structural basis for the unique recognition by such receptors of the triple-helical structure of β1,3-glucan.

PubMed: 21697086
DOI: 10.1074/jbc.M111.256701

実験手法

X-RAY DIFFRACTION (2.2 Å)