3APA

Crystal structure of human pancreatic secretory protein ZG16p

3APA の概要

• エントリーDOI: 10.2210/pdb3apa/pdb
• 関連するPDBエントリー: 3AQG
• 分子名称: Zymogen granule membrane protein 16, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: beta-prism fold, sugar binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted, extracellular space, extracellular matrix: O60844
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15628.95

構造登録者

Kanagawa, M.,Satoh, T.,Nakano, Y.,Kojima-Aikawa, K.,Yamaguchi, Y. (登録日: 2010-10-13, 公開日: 2010-12-08, 最終更新日: 2023-11-01)

主引用文献

Kanagawa, M.,Satoh, T.,Ikeda, A.,Nakano, Y.,Yagi, H.,Kato, K.,Kojima-Aikawa, K.,Yamaguchi, Y.
Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related beta-prism fold
Biochem.Biophys.Res.Commun., 2010

PubMed Abstract: ZG16p is a secretory protein that mediates condensation-sorting of pancreatic enzymes to the zymogen granule membrane in pancreatic acinar cells. ZG16p interacts with glycosaminoglycans and the binding is considered to be important for condensation-sorting of pancreatic enzymes. ZG16b/PAUF, a paralog of ZG16p, has recently been found to play a role in gene regulation and cancer metastasis. However, the detailed functions of ZG16p and ZG16b remain to be clarified. Here, in order to obtain insights into structure-function relationships, we conducted crystallographic studies of human ZG16p lectin as well as its paralog, ZG16b, and determined their crystal structures at 1.65 and 2.75 Å resolution, respectively. ZG16p has a Jacalin-related β-prism fold, the first to be reported among mammalian lectins. The putative sugar-binding site of ZG16p is occupied by a glycerol molecule, mimicking the mannose bound to Jacalin-related mannose-binding-type plant lectins such as Banlec. ZG16b also has a β-prism fold, but some amino acid residues of the putative sugar-binding site differ from those of the mannose-type binding site suggesting altered preference. A positively charged patch, which may bind sulfated groups of the glycosaminoglycans, is located around the putative sugar-binding site of ZG16p and ZG16b. Taken together, we suggest that the sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site.

PubMed: 21110947
DOI: 10.1016/j.bbrc.2010.11.093

実験手法

X-RAY DIFFRACTION (1.65 Å)