3AP5

Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain

3AP5 の概要

• エントリーDOI: 10.2210/pdb3ap5/pdb
• 関連するPDBエントリー: 3AP4 3AP6 3AP7
• 分子名称: Galectin-8 (2 entities in total)
• 機能のキーワード: beta-sandwich, galectin, carbohydrate/sugar binding, sugar binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm (Probable): O00214
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17423.12

構造登録者

Matsuzaka, T.,Ideo, H.,Yamashita, K.,Nonaka, T. (登録日: 2010-10-11, 公開日: 2011-01-26, 最終更新日: 2023-11-01)

主引用文献

Ideo, H.,Matsuzaka, T.,Nonaka, T.,Seko, A.,Yamashita, K.
Galectin-8-N-domain recognition mechanism for sialylated and sulfated glycans
J.Biol.Chem., 286:11346-11355, 2011

PubMed Abstract: Galectin-8 has much higher affinity for 3'-O-sulfated or 3'-O-sialylated glycoconjugates and a Lewis X-containing glycan than for oligosaccharides terminating in Galβ1→3/4GlcNAc, and this specificity is mainly attributed to the N-terminal carbohydrate recognition domain (N-domain, CRD) (Ideo, H., Seko, A., Ishizuka, I., and Yamashita, K. (2003) Glycobiology 13, 713-723). In this study, we elucidated the crystal structures of the human galectin-8-N-domain (-8N) in the absence or presence of 4 ligands. The apo molecule forms a dimer, which is different from the canonical 2-fold symmetric dimer observed for galectin-1 and -2. In a galectin-8N-lactose complex, the lactose-recognizing amino acids are highly conserved among the galectins. However, Arg(45), Gln(47), Arg(59), and the long loop region between the S3 and S4 β-strands are unique to galectin-8N. These amino acids directly or indirectly interact with the sulfate or sialic acid moieties of 3'-sialyl- and 3'-sulfolactose complexed with galectin-8N. Furthermore, in the LNF-III-galectin-8N complex, van der Waals interactions occur between the α1-3-branched fucose and galactose and between galactose and Tyr(141), and these interactions increase the affinity toward galectin-8N. Based on the findings of these x-ray crystallographic analyses, a mutagenesis study using surface plasmon resonance showed that Arg(45), Gln(47), and Arg(59) of galectin-8N are indispensable and coordinately contribute to the strong binding of galectins-8N to sialylated and sulfated oligosaccharides. Arg(59) is the most critical amino acid for binding in the S3-S4 loop region.

PubMed: 21288902
DOI: 10.1074/jbc.M110.195925

実験手法

X-RAY DIFFRACTION (1.92 Å)