3AOT

Crystal structure of juvenile hormone binding protein from silkworm in its apo form

3AOT の概要

• エントリーDOI: 10.2210/pdb3aot/pdb
• 関連するPDBエントリー: 2RQF 3A1Z 3AOS
• 分子名称: Hemolymph juvenile hormone binding protein, TETRAETHYLENE GLYCOL, TRIETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: beta-barrel, juvenile hormone, hemolymph, hormone binding protein
• 由来する生物種: Bombyx mori (silk moth,silkworm)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26220.76

構造登録者

Fujimoto, Z.,Suzuki, R.,Shiotsuki, T.,Momma, M.,Yamazaki, T. (登録日: 2010-10-06, 公開日: 2011-10-12, 最終更新日: 2024-10-09)

主引用文献

Suzuki, R.,Fujimoto, Z.,Shiotsuki, T.,Tsuchiya, W.,Momma, M.,Tase, A.,Miyazawa, M.,Yamazaki, T.
Structural mechanism of JH delivery in hemolymph by JHBP of silkworm, Bombyx mori.
Sci Rep, 1:133-133, 2011

PubMed Abstract: Juvenile hormone (JH) plays crucial roles in many aspects of the insect life. All the JH actions are initiated by transport of JH in the hemolymph as a complex with JH-binding protein (JHBP) to target tissues. Here, we report structural mechanism of JH delivery by JHBP based upon the crystal and solution structures of apo and JH-bound JHBP. In solution, apo-JHBP exists in equilibrium of multiple conformations with different orientations of the gate helix for the hormone-binding pocket ranging from closed to open forms. JH-binding to the gate-open form results in the fully closed JHBP-JH complex structure where the bound JH is completely buried inside the protein. JH-bound JHBP opens the gate helix to release the bound hormone likely by sensing the less polar environment at the membrane surface of target cells. This is the first report that provides structural insight into JH signaling.

PubMed: 22355650
DOI: 10.1038/srep00133

実験手法

X-RAY DIFFRACTION (2.2 Å)