3ALF

Crystal Structure of Class V Chitinase from Nicotiana tobaccum

3ALF の概要

• エントリーDOI: 10.2210/pdb3alf/pdb
• 関連するPDBエントリー: 3ALG
• 分子名称: Chitinase, class V, PHOSPHATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: chitinase, hydrolase
• 由来する生物種: Nicotiana tabacum (tobacco)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39891.92

構造登録者

Numata, T.,Osawa, T.,Ohnuma, T.,Fukamizo, T. (登録日: 2010-08-03, 公開日: 2011-03-23, 最終更新日: 2024-03-13)

主引用文献

Ohnuma, T.,Numata, T.,Osawa, T.,Mizuhara, M.,Varum, K.M.,Fukamizo, T.
Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum
Plant Mol.Biol., 75:291-304, 2011

PubMed Abstract: A class V chitinase from Nicotiana tabacum (NtChiV) with amino acid sequence similar to that of Serratia marcescens chitinase B (SmChiB) was expressed in E. coli and purified to homogeneity. When N-acetylglucosamine oligosaccharides [(NAG)(n)] were hydrolyzed by the purified NtChiV, the second glycosidic linkage from the non-reducing end was predominantly hydrolyzed in a manner similar to that of SmChiB. NtChiV was shown to hydrolyze partially N-acetylated chitosan non-processively, whereas SmChiB hydrolyzes the same substrate processively. The crystal structure of NtChiV was determined by the single-wavelength anomalous dispersion method at 1.2 Å resolution. The protein adopts a classical (β/α)₈-barrel fold (residues 1-233 and 303-348) with an insertion of a small (α + β) domain (residues 234-302). This is the first crystal structure of a plant class V chitinase. The crystal structure of the inactive mutant NtChiV E115Q complexed with (NAG)₄ was also solved and exhibited a linear conformation of the bound oligosaccharide occupying -2, +1, +2, and +3 subsites. The complex structure corresponds to an initial state of (NAG)₄ binding, which is proposed to be converted into a bent conformation through sliding of the +1, +2, and +3 sugar units to -1, +1, and +2 subsites. Although NtChiV is similar to SmChiB, the chitin-binding domain is present in the C-terminus of the latter, but not in the former. Aromatic amino acid residues found in the substrate binding cleft of SmChiB, including Trp97, are substituted with aliphatic residues in NtChiV. These structural differences appear to be responsible for NtChiV being a non-processive enzyme.

PubMed: 21240541
DOI: 10.1007/s11103-010-9727-z

実験手法

X-RAY DIFFRACTION (1.2 Å)