3ALC

ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR DNA-BINDING DOMAIN FROM ASPERGILLUS NIDULANS

3ALC の概要

• エントリーDOI: 10.2210/pdb3alc/pdb
• 分子名称: PROTEIN (ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR), ZINC ION (2 entities in total)
• 機能のキーワード: zinc binuclear cluster, dna-binding, transcriptional activator, dna binding protein
• 由来する生物種: Emericella nidulans
• 細胞内の位置: Nucleus: P21228
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7711.17

構造登録者

Cerdan, R.,Cahuzac, B.,Felenbok, B.,Guittet, E. (登録日: 1999-03-11, 公開日: 2000-05-15, 最終更新日: 2023-12-27)

主引用文献

Cerdan, R.,Cahuzac, B.,Felenbok, B.,Guittet, E.
NMR solution structure of AlcR (1-60) provides insight in the unusual DNA binding properties of this zinc binuclear cluster protein.
J.Mol.Biol., 295:729-736, 2000

PubMed Abstract: The three-dimensional structure of the DNA-binding domain (residues 1-60) of the ethanol regulon transcription factor AlcR from Aspergillus nidulans has been solved by NMR. This domain belongs to the zinc binuclear cluster class. Although the core of the protein is similar to previously characterized structures, consisting of two helices organized around a Zn(2)Cys(6 )motif, the present structure presents important variations, among them the presence of two supplementary helices. This structure gives new insight into the understanding of the AlcR specificities in DNA binding such as longer consensus half-sites, in vitro monomeric binding but in vivo multiple repeat transcriptional activation, either in direct or inverse orientations. The presence of additional contacts of the protein with its DNA target can be predicted from a model proposed for the interaction with the consensus DNA target. The clustering of accessible negative charges on helix 2 delineates a possible interaction site for other determinants of the transcriptional machinery, responsible for the fine tuning of the selection of the AlcR cognate sites.

PubMed: 10656785
DOI: 10.1006/jmbi.1999.3417

実験手法

SOLUTION NMR