3AK9

Crystal structure of the SEp22 dodecamer, a Dps-like protein from Salmonella enterica subsp. enterica serovar Enteritidis, FE-soaked form

Summary for 3AK9

• Entry DOI: 10.2210/pdb3ak9/pdb
• Related: 3AK8
• Descriptor: DNA protection during starvation protein, FE (II) ION, SULFATE ION, ... (5 entities in total)
• Functional Keywords: dps-like protein, dodecamer, iron-binding protein, metal binding protein, oxidoreductase
• Biological source: Salmonella enterica subsp. enterica serovar Kentucky
• Cellular location: Cytoplasm (By similarity): B3YEF4
• Total number of polymer chains: 12
• Total formula weight: 226250.79

Authors

Miyamoto, T.,Asahina, Y.,Miyazaki, S.,Shimizu, H.,Ohto, U.,Noguchi, S.,Satow, Y. (deposition date: 2010-07-08, release date: 2011-01-12, Last modification date: 2023-11-01)

Primary citation

Miyamoto, T.,Asahina, Y.,Miyazaki, S.,Shimizu, H.,Ohto, U.,Noguchi, S.,Satow, Y.
Structures of the SEp22 dodecamer, a Dps-like protein from Salmonella enterica subsp. enterica serovar Enteritidis
Acta Crystallogr.,Sect.F, 67:17-22, 2011

PubMed Abstract: The crystal structure of SEp22, a DNA-binding protein from starved cells from Salmonella enterica subsp. enterica serovar Enteritidis, has been determined in two forms: the native state at 1.25 Å resolution and an iron-soaked form at 1.30 Å resolution. The SEp22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. Along the threefold axes of the 23 symmetry, hydrophilic Asp channels that consist of Asp146 were found. Iron ions may flow into the cavity of the dodecameric shell through the Asp channels.

PubMed: 21206015
DOI: 10.1107/S1744309110043113

Experimental method

X-RAY DIFFRACTION (1.3 Å)