3AJP

Crystal structure of human H ferritin E140A mutant

3AJP の概要

• エントリーDOI: 10.2210/pdb3ajp/pdb
• 関連するPDBエントリー: 3AJO 3AJQ
• 分子名称: Ferritin heavy chain, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: 4-helix bundle, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21309.47

構造登録者

Masuda, T.,Mikami, B. (登録日: 2010-06-11, 公開日: 2010-08-25, 最終更新日: 2024-04-03)

主引用文献

Masuda, T.,Goto, F.,Yoshihara, T.,Mikami, B.
The universal mechanism for iron translocation to the ferroxidase site in ferritin, which is mediated by the well conserved transit site
Biochem.Biophys.Res.Commun., 400:94-99, 2010

PubMed Abstract: Ferritins are ubiquitous iron storage proteins. Recently, we identified a novel metal-binding site, transit site, in the crystal structure of phytoferritin. To elucidate the function of the transit site in ferritin from other species, we prepared transit-site-deficient mutants of human H ferritin, E140A and E140Q, and their iron oxidation kinetics was analyzed. The initial velocities of iron oxidization were reduced in the variants, especially in E140Q. The crystal structure of E140Q showed that the side chain of the mutated Gln140 was fixed by a hydrogen bond, whereas that of native Glu140 was flexible. These results suggest that the conserved transit site also has a function to assist with the metal ion sequestration to the ferroxidase site in ferritins from vertebrates.

PubMed: 20705053
DOI: 10.1016/j.bbrc.2010.08.017

実験手法

X-RAY DIFFRACTION (1.901 Å)