3AJK

Crystal structure of d(CGCGGATf5UCGCG): 5-Formyluridine:Guanosine Base-pair in B-DNA with Hoechst33258

3AJK の概要

• エントリーDOI: 10.2210/pdb3ajk/pdb
• 関連するPDBエントリー: 3AJJ 3AJL
• 分子名称: 5'-D(*CP*GP*CP*GP*GP*AP*TP*(UFR)P*CP*GP*CP*G*)-3', 2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7835.55

構造登録者

Tsunoda, M.,Sakaue, T.,Ueno, Y.,Matsuda, A.,Takenaka, A. (登録日: 2010-06-07, 公開日: 2011-04-27, 最終更新日: 2023-11-01)

主引用文献

Tsunoda, M.,Sakaue, T.,Naito, S.,Sunami, T.,Abe, N.,Ueno, Y.,Matsuda, A.,Takenaka, A.
Insights into the structures of DNA damaged by hydroxyl radical: crystal structures of DNA duplexes containing 5-formyluracil
J Nucleic Acids, 2010:107289-107289, 2010

PubMed Abstract: Hydroxyl radicals are potent mutagens that attack DNA to form various base and ribose derivatives. One of the major damaged thymine derivatives is 5-formyluracil (fU), which induces pyrimidine transition during replication. In order to establish the structural basis for such mutagenesis, the crystal structures of two kinds of DNA d(CGCGRATfUCGCG) with R = A/G have been determined by X-ray crystallography. The fU residues form a Watson-Crick-type pair with A and two types of pairs (wobble and reversed wobble) with G, the latter being a new type of base pair between ionized thymine base and guanine base. In silico structural modeling suggests that the DNA polymerase can accept the reversed wobble pair with G, as well as the Watson-Crick pair with A.

PubMed: 20976303
DOI: 10.4061/2010/107289

実験手法

X-RAY DIFFRACTION (1.95 Å)