3AJF

Structural insigths into dsRNA binding and RNA silencing suppression by NS3 protein of rice hoja blanca tenuivirus

3AJF の概要

• エントリーDOI: 10.2210/pdb3ajf/pdb
• 分子名称: Non-structural protein 3, SULFATE ION (3 entities in total)
• 機能のキーワード: rna silencing suppression, negative strand rna virus, viral protein
• 由来する生物種: Rice hoja blanca virus
• 細胞内の位置: Host cytoplasm (By similarity): Q67897
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45510.72

構造登録者

Yuan, Y.A. (登録日: 2010-06-05, 公開日: 2011-04-20, 最終更新日: 2024-10-16)

主引用文献

Yang, X.,Tan, S.H.,Teh, Y.J.,Yuan, Y.A.
Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus
Rna, 17:903-911, 2011

PubMed Abstract: Rice Hoja Blanca Tenuivirus (RHBV), a negative strand RNA virus, has been identified to infect rice and is widely transmitted by the insect vector. NS3 protein encoded by RHBV RNA3 was reported to be a potent RNAi suppressor to counterdefense RNA silencing in plants, insect cells, and mammalian cells. Here, we report the crystal structure of the N-terminal domain of RHBV NS3 (residues 21-114) at 2.0 Å. RHBV NS3 N-terminal domain forms a dimer by two pairs of α-helices in an anti-parallel mode, with one surface harboring a shallow groove at the dimension of 20 Å × 30 Å for putative dsRNA binding. In vitro RNA binding assay and RNA silencing suppression assay have demonstrated that the structural conserved residues located along this shallow groove, such as Arg50, His51, Lys77, and His85, participate in dsRNA binding and RNA silencing suppression. Our results provide the initial structural implications in understanding the RNAi suppression mechanism by RHBV NS3.

PubMed: 21460234
DOI: 10.1261/rna.2552811

実験手法

X-RAY DIFFRACTION (2 Å)