3AIX

Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)

Summary for 3AIX

• Entry DOI: 10.2210/pdb3aix/pdb
• Related: 3AIZ
• Descriptor: DNA polymerase sliding clamp C, DNA polymerase sliding clamp B, SULFATE ION, ... (4 entities in total)
• Functional Keywords: protein-protein complex, replication
• Biological source: Sulfolobus tokodaii; More
• Total number of polymer chains: 2
• Total formula weight: 55322.42

Authors

Kawai, A.,Higuchi, S.,Miyamoto, S. (deposition date: 2010-05-18, release date: 2011-05-18, Last modification date: 2023-11-01)

Primary citation

Kawai, A.,Hashimoto, H.,Higuchi, S.,Tsunoda, M.,Sato, M.,Nakamura, K.T.,Miyamoto, S.
A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex
J.Struct.Biol., 174:443-450, 2011

PubMed Abstract: Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2-stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2-stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.

PubMed: 21352919
DOI: 10.1016/j.jsb.2011.02.006

Experimental method

X-RAY DIFFRACTION (2.9 Å)