3AG7

An auxilin-like J-domain containing protein, JAC1 J-domain

Summary for 3AG7

• Entry DOI: 10.2210/pdb3ag7/pdb
• Related: 1nz6 2qwn 2qwo 2qwp 2qwq 2qwr
• Descriptor: Putative uncharacterized protein F9E10.5 (2 entities in total)
• Functional Keywords: j-domain, an auxilin-like j-domain containing protein, jac1, chloroplast accumulation response, plant protein
• Biological source: Arabidopsis thaliana (mouse-ear cress, thale-cress)
• Total number of polymer chains: 1
• Total formula weight: 11940.75

Authors

Takano, A. (deposition date: 2010-03-24, release date: 2010-10-13, Last modification date: 2023-11-01)

Primary citation

Takano, A.,Suetsugu, N.,Wada, M.,Kohda, D.
Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photorelocation movement in Arabidopsis thaliana
Plant Cell.Physiol., 51:1372-1376, 2010

PubMed Abstract: An auxilin-like J-domain-containing protein, JAC1, is necessary for chloroplast movement in Arabidopsis thaliana, to capture photosynthetic light efficiently under weak light conditions. Here, we performed crystallographic and functional analyses of the J-domain of JAC1. The crystal structure of the J-domain is quite similar to that of bovine auxilin, and possesses a similar positively charged surface, which probably forms the interface with the Hsp70 chaperone. The mutation of the highly conserved HPD motif of the JAC1 J-domain abrogated the chloroplast photorelocation response. These results suggest that the requirement of JAC1 in chloroplast photorelocation movement is attributable to the J-domain's cochaperone activity.

PubMed: 20562448
DOI: 10.1093/pcp/pcq089

Experimental method

X-RAY DIFFRACTION (1.8 Å)