3AFP

Crystal structure of the single-stranded DNA binding protein from Mycobacterium leprae (Form I)

3AFP の概要

• エントリーDOI: 10.2210/pdb3afp/pdb
• 関連するPDBエントリー: 3AFQ
• 分子名称: Single-stranded DNA-binding protein, GLYCEROL, CADMIUM ION, ... (4 entities in total)
• 機能のキーワード: ob-fold, quaternary structure and stability, changes on oligomerisation, water-bridges, dna damage, dna repair, dna replication, dna-binding, dna binding protein
• 由来する生物種: Mycobacterium leprae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35643.50

構造登録者

Kaushal, P.S.,Singh, P.,Sharma, A.,Muniyappa, K.,Vijayan, M. (登録日: 2010-03-10, 公開日: 2010-10-06, 最終更新日: 2023-11-01)

主引用文献

Kaushal, P.S.,Singh, P.,Sharma, A.,Muniyappa, K.,Vijayan, M.
X-ray and molecular-dynamics studies on Mycobacterium leprae single-stranded DNA-binding protein and comparison with other eubacterial SSB structures
Acta Crystallogr.,Sect.D, 66:1048-1058, 2010

PubMed Abstract: The crystal structures of two forms of Mycobacterium leprae single-stranded DNA-binding protein (SSB) have been determined at 2.05 and 2.8 Å resolution. Comparison of these structures with the structures of other eubacterial SSBs indicates considerable variation in their quaternary association, although the DNA-binding domains in all of them exhibit the same OB-fold. This variation has no linear correlation with sequence variation, but could be related to variation in protein stability. Molecular-dynamics simulations have been carried out on tetrameric molecules derived from the two forms and the prototype Escherichia coli SSB and the individual subunits of both proteins. Together, the X-ray studies and molecular-dynamics simulations yield information on the relatively rigid and flexible regions of the molecule and on the effect of oligomerization on flexibility. The simulations provide insight into the changes in subunit structure on oligomerization. They also provide insight into the stability and time evolution of the hydrogen bonds/water bridges that connect the two pairs of monomers in the tetramer.

PubMed: 20944238
DOI: 10.1107/S0907444910032208

実験手法

X-RAY DIFFRACTION (2.05 Å)