3AFA

The human nucleosome structure

3AFA の概要

• エントリーDOI: 10.2210/pdb3afa/pdb
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (8 entities in total)
• 機能のキーワード: histone-fold, dna-binding protein, chromosomal protein, citrullination, dna-binding, methylation, nucleosome core, nucleus, phosphoprotein, isopeptide bond, antibiotic, antimicrobial, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P68431 P62805 P04908 P06899
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202812.00

構造登録者

Tachiwana, H.,Kagawa, W.,Osakabe, A.,Koichiro, K.,Shiga, T.,Kimura, H.,Kurumizaka, H. (登録日: 2010-02-24, 公開日: 2010-05-26, 最終更新日: 2023-11-01)

主引用文献

Tachiwana, H.,Kagawa, W.,Osakabe, A.,Kawaguchi, K.,Shiga, T.,Hayashi-Takanaka, Y.,Kimura, H.,Kurumizaka, H.
Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T
Proc.Natl.Acad.Sci.USA, 107:10454-10459, 2010

PubMed Abstract: A histone H3 variant, H3T, is highly expressed in the testis, suggesting that it may play an important role in the chromatin reorganization required for meiosis and/or spermatogenesis. In the present study, we found that the nucleosome containing human H3T is significantly unstable both in vitro and in vivo, as compared to the conventional nucleosome containing H3.1. The crystal structure of the H3T nucleosome revealed structural differences in the H3T regions on both ends of the central alpha2 helix, as compared to those of H3.1. The H3T-specific residues (Met71 and Val111) are the source of the structural differences observed between H3T and H3.1. A mutational analysis revealed that these residues are responsible for the reduced stability of the H3T-containing nucleosome. These physical and structural properties of the H3T-containing nucleosome may provide the basis of chromatin reorganization during spermatogenesis.

PubMed: 20498094
DOI: 10.1073/pnas.1003064107

実験手法

X-RAY DIFFRACTION (2.5 Å)