3ACO

Crystal structure of the EFC/F-BAR domain of human PACSIN2/Syndapin II (2.7 A)

Summary for 3ACO

• Entry DOI: 10.2210/pdb3aco/pdb
• Related: 3ABH
• Descriptor: Protein kinase C and casein kinase substrate in neurons protein 2, CALCIUM ION (3 entities in total)
• Functional Keywords: helix bundle, coiled-coil, endocytosis
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm : Q9UNF0
• Total number of polymer chains: 2
• Total formula weight: 82141.38

Authors

Shimada, A.,Shirouzu, M.,Hanawa-Suetsugu, K.,Terada, T.,Umehara, T.,Suetsugu, S.,Yamamoto, M.,Yokoyama, S. (deposition date: 2010-01-07, release date: 2010-04-14, Last modification date: 2024-10-23)

Primary citation

Shimada, A.,Takano, K.,Shirouzu, M.,Hanawa-Suetsugu, K.,Terada, T.,Toyooka, K.,Umehara, T.,Yamamoto, M.,Yokoyama, S.,Suetsugu, S.
Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II
Febs Lett., 584:1111-1118, 2010

PubMed Abstract: The extended Fes-CIP4 homology (EFC)/FCH-BAR (F-BAR) domain tubulates membranes. Overexpression of the pacsin2 EFC/F-BAR domain resulted in tubular localization inside cells and deformed liposomes into tubules in vitro. We found that overexpression of the pacsin2 EFC/F-BAR domain induced cellular microspikes, with the pacsin2 EFC/F-BAR domain concentrated at the neck. The hydrophobic loops and the basic amino-acid residues on the concave surface of the pacsin2 EFC/F-BAR domain are essential for both the microspike formation and tubulation. Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate both microspike formation and tubulation.

PubMed: 20188097
DOI: 10.1016/j.febslet.2010.02.058

Experimental method

X-RAY DIFFRACTION (2.7 Å)