3ACF

Crystal Structure of Carbohydrate-Binding Module Family 28 from Clostridium josui Cel5A in a ligand-free form

Summary for 3ACF

• Entry DOI: 10.2210/pdb3acf/pdb
• Related: 3ACG 3ACH 3ACI
• Descriptor: Beta-1,4-endoglucanase, CALCIUM ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: beta-jellyroll, cellulose-binding domain, glycosidase, hydrolase
• Biological source: Clostridium josui
• Total number of polymer chains: 1
• Total formula weight: 22526.33

Authors

Tsukimoto, K.,Takada, R.,Araki, Y.,Suzuki, K.,Karita, S.,Wakagi, T.,Shoun, H.,Watanabe, T.,Fushinobu, S. (deposition date: 2010-01-04, release date: 2010-03-02, Last modification date: 2023-11-01)

Primary citation

Tsukimoto, K.,Takada, R.,Araki, Y.,Suzuki, K.,Karita, S.,Wakagi, T.,Shoun, H.,Watanabe, T.,Fushinobu, S.
Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module.
Febs Lett., 584:1205-1211, 2010

PubMed Abstract: The crystal structures of a carbohydrate-binding module (CBM) family 28 domain of endoglucanase Cel5A from Clostridium josui have been determined in ligand-free and complex forms with cellobiose, cellotetraose, and cellopentaose as the first complex structures of this family. In the cleft of a beta-sandwich fold, the ligands are recognized by stacking interactions and hydrogen bonds. Conformations of the bound cellooligosaccharides are similar to those in crystals and solution but clearly different from the cellulose structure. Interestingly, the glucan chain bound on CBM28 is in the opposite direction of that bound to CBM17, although these families share significant structural similarity.

PubMed: 20159017
DOI: 10.1016/j.febslet.2010.02.027

Experimental method

X-RAY DIFFRACTION (1.6 Å)