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3ABX

CcCel6C, a glycoside hydrolase family 6 enzyme, complexed with p-nitrophenyl beta-D-cellotrioside

3A9A」から置き換えられました
3ABX の概要
エントリーDOI10.2210/pdb3abx/pdb
関連するPDBエントリー3A64 3A9B
分子名称Cellobiohydrolase, MAGNESIUM ION, 4-nitrophenyl beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranoside, ... (4 entities in total)
機能のキーワードseven-stranded beta-alpha barrel, cellulase, glycoside hydrolase family 6, cellobiohydrolase, hydrolase
由来する生物種Coprinopsis cinerea (Inky cap fungus)
タンパク質・核酸の鎖数1
化学式量合計44906.47
構造登録者
Liu, Y.,Yoshida, M.,Kurakata, Y.,Miyazaki, T.,Nishikawa, A.,Tonozuka, T. (登録日: 2009-12-24, 公開日: 2010-01-05, 最終更新日: 2024-11-20)
主引用文献Liu, Y.,Yoshida, M.,Kurakata, Y.,Miyazaki, T.,Igarashi, K.,Samejima, M.,Fukuda, K.,Nishikawa, A.,Tonozuka, T.
Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea
Febs J., 277:1532-1542, 2010
Cited by
PubMed Abstract: The basidiomycete Coprinopsis cinerea produces the glycoside hydrolase family 6 enzyme CcCel6C at low and constitutive levels. CcCel6C exhibits unusual cellobiohydrolase activity; it hydrolyses carboxymethyl cellulose, which is a poor substrate for typical cellobiohydrolases. Here, we determined the crystal structures of CcCel6C unbound and in complex with p-nitrophenyl beta-D-cellotrioside and cellobiose. CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, which is observed in other cellobiohydrolases from ascomecetes Hypocrea jecorina (HjeCel6A) and Humicola insolens (HinCel6A). In HjeCel6A and HinCel6A, ligand binding produces a conformational change that narrows this tunnel. In contrast, the tunnel remains wide in CcCel6C and the conformational change appears to be less favourable than in HjeCel6A and HinCel6A. The ligand binding cleft for subsite -3 of CcCel6C is also wide and is rather similar to that of endoglucanase. These results suggest that the open tunnel and the wide cleft are suitable for the hydrolysis of carboxymethyl cellulose.
PubMed: 20148970
DOI: 10.1111/j.1742-4658.2010.07582.x
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.4 Å)
構造検証レポート
Validation report summary of 3abx
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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