3AB6

Crystal structure of NAG3 bound lysozyme from Meretrix lusoria

3AB6 の概要

• エントリーDOI: 10.2210/pdb3ab6/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Lysozyme, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: lysozyme, meretrix lusoria, antibiotic, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase, secreted
• 由来する生物種: Meretrix lusoria (Hard clam)
• 細胞内の位置: Secreted : P86383
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14012.11

構造登録者

Yoneda, K.,Kuwano, Y.,Araki, T. (登録日: 2009-12-01, 公開日: 2010-12-01, 最終更新日: 2024-10-16)

主引用文献

Kuwano, Y.,Yoneda, K.,Kawaguchi, Y.,Araki, T.
The tertiary structure of an i-type lysozyme isolated from the common orient clam (Meretrix lusoria)
Acta Crystallogr.,Sect.F, 69:1202-1206, 2013

PubMed Abstract: To evaluate the structure-function relationships of invertebrate lysozymes, a new invertebrate-type (i-type) lysozyme was isolated from the common orient clam (Meretrix lusoria) and the tertiary structure of this enzyme was determined. Comparison of the tertiary structure of this enzyme with those of chicken and Venerupi philippinarum lysozymes revealed that the location of the side chain of the second catalytic residue, an aspartic acid, and the N-acetylglucosamine trimer bound at subsites A-C were different. Furthermore, the amino acid electrostatically interacting with Asp30 in V. philippinarum lysozyme, Lys108, was substituted by Gly in M. lusoria lysozyme and no other possible amino acid that could contribute to this interaction was found in M. lusoria lysozyme. It therefore seems that the substitutions of the amino acids at the interface of the V. philippinarum lysozyme dimer are likely to change the oligomeric state of the M. lusoria lysozyme.

PubMed: 24192349
DOI: 10.1107/S1744309113028170

実験手法

X-RAY DIFFRACTION (1.78 Å)