3AAQ

Crystal structure of Lp1NTPDase from Legionella pneumophila in complex with the inhibitor ARL 67156

3AAQ の概要

• エントリーDOI: 10.2210/pdb3aaq/pdb
• 関連するPDBエントリー: 3AAP 3AAR
• 分子名称: Ectonucleoside triphosphate diphosphohydrolase I, 5'-O-[(R)-{[(R)-[dibromo(phosphono)methyl](hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-N,N-diethyladenosine (3 entities in total)
• 機能のキーワード: adenosine triphosphatase, ntpdase, hydrolase
• 由来する生物種: Legionella pneumophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40751.73

構造登録者

Vivian, J.P.,Beddoe, T.,Rossjohn, J. (登録日: 2009-11-24, 公開日: 2010-02-09, 最終更新日: 2024-11-06)

主引用文献

Vivian, J.P.,Riedmaier, P.,Ge, H.,Le Nours, J.,Sansom, F.M.,Wilce, M.C.J.,Byres, E.,Dias, M.,Schmidberger, J.W.,Cowan, P.J.,d'Apice, A.J.F.,Hartland, E.L.,Rossjohn, J.,Beddoe, T.
Crystal Structure of a Legionella pneumophila Ecto -Triphosphate Diphosphohydrolase, A Structural and Functional Homolog of the Eukaryotic NTPDases
Structure, 18:228-238, 2010

PubMed Abstract: Many pathogenic bacteria have sophisticated mechanisms to interfere with the mammalian immune response. These include the disruption of host extracellular ATP levels that, in humans, is tightly regulated by the nucleoside triphosphate diphosphohydrolase family (NTPDases). NTPDases are found almost exclusively in eukaryotes, the notable exception being their presence in some pathogenic prokaryotes. To address the function of bacterial NTPDases, we describe the structures of an NTPDase from the pathogen Legionella pneumophila (Lpg1905/Lp1NTPDase) in its apo state and in complex with the ATP analog AMPPNP and the subtype-specific NTPDase inhibitor ARL 67156. Lp1NTPDase is structurally and catalytically related to eukaryotic NTPDases and the structure provides a basis for NTPDase-specific inhibition. Furthermore, we demonstrate that the activity of Lp1NTPDase correlates directly with intracellular replication of Legionella within macrophages. Collectively, these findings provide insight into the mechanism of this enzyme and highlight its role in host-pathogen interactions.

PubMed: 20159467
DOI: 10.1016/j.str.2009.11.014

実験手法

X-RAY DIFFRACTION (2 Å)