3A64

Crystal structure of CcCel6C, a glycoside hydrolase family 6 enzyme, from Coprinopsis cinerea

3A64 の概要

• エントリーDOI: 10.2210/pdb3a64/pdb
• 分子名称: Cellobiohydrolase, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: seven-stranded beta-alpha barrel, cellulase, glycoside hydrolase family 6, cellobiohydrolase, hydrolase
• 由来する生物種: Coprinopsis cinerea (Inky cap fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43655.41

構造登録者

Liu, Y.,Yoshida, M.,Kurakata, Y.,Miyazaki, T.,Nishikawa, A.,Tonozuka, T. (登録日: 2009-08-21, 公開日: 2009-09-01, 最終更新日: 2024-11-20)

主引用文献

Liu, Y.,Yoshida, M.,Kurakata, Y.,Miyazaki, T.,Igarashi, K.,Samejima, M.,Fukuda, K.,Nishikawa, A.,Tonozuka, T.
Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea
Febs J., 277:1532-1542, 2010

PubMed Abstract: The basidiomycete Coprinopsis cinerea produces the glycoside hydrolase family 6 enzyme CcCel6C at low and constitutive levels. CcCel6C exhibits unusual cellobiohydrolase activity; it hydrolyses carboxymethyl cellulose, which is a poor substrate for typical cellobiohydrolases. Here, we determined the crystal structures of CcCel6C unbound and in complex with p-nitrophenyl beta-D-cellotrioside and cellobiose. CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, which is observed in other cellobiohydrolases from ascomecetes Hypocrea jecorina (HjeCel6A) and Humicola insolens (HinCel6A). In HjeCel6A and HinCel6A, ligand binding produces a conformational change that narrows this tunnel. In contrast, the tunnel remains wide in CcCel6C and the conformational change appears to be less favourable than in HjeCel6A and HinCel6A. The ligand binding cleft for subsite -3 of CcCel6C is also wide and is rather similar to that of endoglucanase. These results suggest that the open tunnel and the wide cleft are suitable for the hydrolysis of carboxymethyl cellulose.

PubMed: 20148970
DOI: 10.1111/j.1742-4658.2010.07582.x

実験手法

X-RAY DIFFRACTION (1.6 Å)