3A52

Crystal structure of cold-active alkailne phosphatase from psychrophile Shewanella sp.

3A52 の概要

• エントリーDOI: 10.2210/pdb3a52/pdb
• 分子名称: Cold-active alkaline phosphatase, SULFATE ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: phosphatase, hydrolase
• 由来する生物種: Shewanella
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86808.94

構造登録者

Tsuruta, H.,Mikami, B.,Higashi, T.,Aizono, Y. (登録日: 2009-07-24, 公開日: 2010-04-14, 最終更新日: 2024-03-13)

主引用文献

Tsuruta, H.,Mikami, B.,Higashi, T.,Aizono, Y.
Crystal structure of cold-active alkaline phosphatase from the psychrophile Shewanella sp.
Biosci.Biotechnol.Biochem., 74:69-74, 2010

PubMed Abstract: The crystal structure of a cold-active alkaline phosphatase from a psychrophile, Shewanella sp. (SCAP), was solved at 2.2 A. A refined model showed a homodimer with six metal-ligand sites. The arrangement of the catalytic residues resembled those of alkaline phosphatases (APs), suggesting that the reaction mechanism of SCAP was fundamentally identical to those of other APs. SCAP had two distinct structural features: (i) a loop with Arg122 that bound to the phosphate moiety of the substrate suffered no constraints from the linkage to other secondary structures, and (ii) Mg3-ligand His109 was considered to undergo repulsive effect with neighboring Trp228. The local flexibility led by these features might be an important factor in the high catalytic efficiency of SCAP at low temperatures.

PubMed: 20057143
DOI: 10.1271/bbb.90563

実験手法

X-RAY DIFFRACTION (2.2 Å)