3A0T

Catalytic domain of histidine kinase ThkA (TM1359) in complex with ADP and Mg ion (trigonal)

3A0T の概要

• エントリーDOI: 10.2210/pdb3a0t/pdb
• 関連するPDBエントリー: 3A0R 3A0S
• 分子名称: Sensor protein, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: atp-lid, kinase, phosphoprotein, transferase, two-component regulatory system
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18177.74

構造登録者

Yamada, S.,Sugimoto, H.,Kobayashi, M.,Ohno, A.,Nakamura, H.,Shiro, Y. (登録日: 2009-03-24, 公開日: 2009-10-20, 最終更新日: 2024-04-03)

主引用文献

Yamada, S.,Sugimoto, H.,Kobayashi, M.,Ohno, A.,Nakamura, H.,Shiro, Y.
Structure of PAS-linked histidine kinase and the response regulator complex
Structure, 17:1333-1344, 2009

PubMed Abstract: We determined the structure of the complex of the sensory histidine kinase (HK) and its cognate response regulator (RR) in the two-component signal transduction system of Thermotoga maritima. This was accomplished by fitting the high-resolution structures of the isolated HK domains and the RR onto the electron density map (3.8 A resolution) of the HK/RR complex crystal. Based on the structural information, we evaluated the roles of both interdomain and intermolecular interactions in the signal transduction of the cytosolic PAS-linked HK and RR system, in particular the O(2)-sensor FixL/FixJ system. The PAS-sensor domain of HK interacts with the catalytic domain of the same polypeptide chain by creating an interdomain beta sheet. The interaction site between HK and RR, which was confirmed by NMR, is suitable for the intermolecular transfer reaction of the phosphoryl group, indicating that the observed interaction is important for the phosphatase activity of HK that dephosphorylates phospho-RR.

PubMed: 19836334
DOI: 10.1016/j.str.2009.07.016

実験手法

X-RAY DIFFRACTION (1.91 Å)