3A03

Crystal structure of Hox11L1 homeodomain

3A03 の概要

• エントリーDOI: 10.2210/pdb3a03/pdb
• 関連するPDBエントリー: 3A01 3A02
• 分子名称: T-cell leukemia homeobox protein 2, SODIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: homeodomain, developmental protein, dna-binding, homeobox, nucleus, gene regulation
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus : O43763
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7047.05

構造登録者

Miyazono, K.,Nagata, K.,Saigo, K.,Kojima, T.,Tanokura, M. (登録日: 2009-02-28, 公開日: 2010-03-09, 最終更新日: 2024-03-13)

主引用文献

Miyazono, K.,Zhi, Y.,Takamura, Y.,Nagata, K.,Saigo, K.,Kojima, T.,Tanokura, M.
Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless
Embo J., 29:1613-1623, 2010

PubMed Abstract: To achieve accurate gene regulation, some homeodomain proteins bind cooperatively to DNA to increase those site specificities. We report a ternary complex structure containing two homeodomain proteins, aristaless (Al) and clawless (Cll), bound to DNA. Our results show that the extended conserved sequences of the Cll homeodomain are indispensable to cooperative DNA binding. In the Al-Cll-DNA complex structure, the residues in the extended regions are used not only for the intermolecular contacts between the two homeodomain proteins but also for the sequence-recognition mechanism of DNA by direct interactions. The residues in the extended N-terminal arm lie within the minor groove of DNA to form direct interactions with bases, whereas the extended conserved region of the C-terminus of the homeodomain interacts with Al to stabilize and localize the third alpha helix of the Cll homeodomain. This structure suggests a novel mode for the cooperativity of homeodomain proteins.

PubMed: 20389279
DOI: 10.1038/emboj.2010.53

実験手法

X-RAY DIFFRACTION (1.54 Å)