398D

3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION

398D の概要

• エントリーDOI: 10.2210/pdb398d/pdb
• 分子名称: RNA (5'-R(*GP*CP*AP*GP*UP*GP*GP*C)-3'), DNA/RNA (5'-R(*GP*CP*CP*AP)-D(*CP*TP*GP*C)-3') (3 entities in total)
• 機能のキーワード: a-dna/rna, double helix, dna-rna hybrid
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 10066.36

構造登録者

Mueller, U.,Meier, G.,Mochi-Onori, A.,Cellai, L.,Heumann, H. (登録日: 1998-05-04, 公開日: 1998-10-06, 最終更新日: 2024-04-03)

主引用文献

Mueller, U.,Maier, G.,Mochi Onori, A.,Cellai, L.,Heumann, H.,Heinemann, U.
Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication.
Biochemistry, 37:12005-12011, 1998

PubMed Abstract: During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.

PubMed: 9724510
DOI: 10.1021/bi981152y

実験手法

X-RAY DIFFRACTION (1.94 Å)