362D

THE STRUCTURE OF D(TGCGCA)2 AND A COMPARISON TO OTHER Z-DNA HEXAMERS

「347D」から置き換えられました

362D の概要

• エントリーDOI: 10.2210/pdb362d/pdb
• 分子名称: DNA (5'-D(*TP*GP*CP*GP*CP*A)-3'), COBALT HEXAMMINE(III) (3 entities in total)
• 機能のキーワード: z-dna, double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3940.67

構造登録者

Harper, N.A.,Brannigan, J.A.,Buck, M.,Lewis, R.J.,Moore, M.H.,Schneider, B. (登録日: 1997-08-20, 公開日: 1997-11-20, 最終更新日: 2023-08-02)

主引用文献

Harper, A.,Brannigan, J.A.,Buck, M.,Hewitt, L.,Lewis, R.J.,Moore, M.H.,Schneider, B.
Structure of d(TGCGCA)2 and a comparison to other DNA hexamers.
Acta Crystallogr.,Sect.D, 54:1273-1284, 1998

PubMed Abstract: The X-ray crystal structure of d(TGCGCA)2 has been determined at 120 K to a resolution of 1.3 A. Hexamer duplexes, in the Z-DNA conformation, pack in an arrangement similar to the 'pure spermine form' [Egli et al. (1991). Biochemistry, 30, 11388-11402] but with significantly different cell dimensions. The phosphate backbone exists in two equally populated discrete conformations at one nucleotide step, around phosphate 11. The structure contains two ordered cobalt hexammine molecules which have roles in stabilization of both the Z-DNA conformation of the duplex and in crystal packing. A comparison of d(TGCGCA)2 with other Z-DNA hexamer structures available in the Nucleic Acid Database illustrates the elusive nature of crystal packing. A review of the interactions with the metal cations Na+, Mg2+ and Co3+ reveals a relatively small proportion of phosphate binding and that close contacts between metal ions are common. A prediction of the water structure is compared with the observed pattern in the reported structure.

PubMed: 10089504
DOI: 10.1107/S0907444998002807

実験手法

X-RAY DIFFRACTION (1.3 Å)