359D

INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)

359D の概要

• エントリーDOI: 10.2210/pdb359d/pdb
• 分子名称: RNA HAMMERHEAD RIBOZYME, TERBIUM(III) ION (3 entities in total)
• 機能のキーワード: rna hammerhead ribozyme, catalytic rna, loop, ribozyme
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13984.60

構造登録者

Feig, A.L.,Scott, W.G.,Uhlenbeck, O.C. (登録日: 1997-10-27, 公開日: 1997-11-07, 最終更新日: 2023-08-02)

主引用文献

Feig, A.L.,Scott, W.G.,Uhlenbeck, O.C.
Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb.
Science, 279:81-84, 1998

PubMed Abstract: Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.

PubMed: 9417029
DOI: 10.1126/science.279.5347.81

実験手法

X-RAY DIFFRACTION (2.9 Å)